Abstract

RUBIO-TINAJERO, Sarahi et al. In vitro antagonist biocontrol of Fusarium oxysporum and Dickeya chrysanthemi. Rev. mex. fitopatol [online]. 2021, vol.39, n.3, pp.515-528.  Epub Dec 13, 2021. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.2104-1.

The objective was to evaluate the antagonistic effect in vitro of native strains of Trichoderma asperellum, Trichoderma harzianum and two isolates of Bacillus spp. against Fusarium oxysporum and Dickeya chrysanthemi. Bacillus, isolated from soil samples of Aloe vera plantations, was morphologically identified and a modified dual culture confrontation was carried out, placing the F. oxysporum in the center of the Petri dish and Bacillus in the four cardinal points; it was arranged under a completely randomized experimental design with five repetitions, the variables registered were percentage of antagonism, inhibition halo and concentration of conidia. For Trichoderma they were confronted against F. oxysporum and D. chrysanthemi with a dual confrontation, a percentage of inhibition and antagonism classification were determined. In the confrontation of T. asperellum it obtained a percentage of inhibition of 70.5% against F. oxysporum and 41.9% against D. chrysanthemi. Regarding the inhibition halos of Bacillus (B5 and B4), they obtained 4 mm. In the conidia concentration B5 showed 1.3, B4 2.6 and the control 12.6 spores per dilution. Trichoderma and Bacillus represent a viable alternative for the control of F. oxysporum and D. chrysanthemi.

Keywords : Biological control; fungus; bacteria; T. asperellum; T. harzianum; Bacillus spp.

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