Abstract

RIOS-HERNANDEZ, Tania Ameyally; UC-VARGUEZ, Alberto  and  EVANGELISTA-MARTINEZ, Zahaed. Biological control of Fusarium oxysporum causal agent of gladiolus corm rot by streptomycetes. Rev. mex. fitopatol [online]. 2021, vol.39, n.3, pp.391-413.  Epub Dec 13, 2021. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.2105-3.

Fusarium oxysporum causes gladiolus corm rot and production damage could reach up to 100%. Fusarium isolates were selected from infected corms, one of them was morphologically and molecularly identified, and was selected from pathogenicity testing. One strain from 22 streptomycetes isolates that showed a 40% antagonist activity against Fusarium was selected. Bioactive extract (BE) was obtained by Solid Phase Fermentation and the minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) were determined by the microdilution method. A MIC of 0.19 mg mL-1 and an MLC of 0.38 mg mL-1 were obtained, which was confirmed with a conidia germination test at 8 h, which showed inhibition percentage of 17 and 98% for ¼ and ½ of the MIC. The effect of BE was evaluated at 1 and 2 MIC’s concentration against corm rot in infected gladiolus corm, obtaining a protective effect of gladiolus corms and maintaining their hardness after 15 days, in comparison with the fungicide Carbendazim. These results indicate Streptomyces sp., as a potential biological control agent against F. oxysporum.

Keywords : Gladiolus grandiflorus; Actinobacterias; fungal activity; corms; protective effect.

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