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Revista de investigación clínica
On-line version ISSN 2564-8896Print version ISSN 0034-8376
Abstract
CUELLAR-GUEVARA, Fabiola L.; BARRON-GONZALEZ, María P. and MENCHACA-ARREDONDO, Jorge L.. Effect of Lactobacillus Postbiotics on Entamoeba Histolytica Trophozoites. Rev. invest. clín. [online]. 2019, vol.71, n.6, pp.402-407. Epub Apr 09, 2021. ISSN 2564-8896. https://doi.org/10.24875/ric.19003134.
Background
Amebiasis is an infectious disease caused by Entamoeba histolytica. It represents one of the three worldwide leading causes of death by parasites and a public health problem due to its frequency, morbidity, mortality, and easy dispersion.
Objective
The study was aimed to evaluate the in vitro effect of Lactobacillus spp. postbiotics on E. histolytica trophozoites (HM1-IMSS strain) and to determine morphometric changes in trophozoite membrane by atomic force microscopy (AFM).
Methods
Bioassays on trophozoites were conducted with lyophilized postbiotics at 0.1, 0.3, and 0.5 mg/mL concentrations, and trophozoite samples were obtained for AFM analysis
Results
Results indicated postbiotic inhibitory activity; the highest percentage inhibition was 89.63% at 0.5 mg/mL. Trophozoites nanomechanical analysis showed 28.32% increase in ruggedness and 56% decrease in size with treatments compared to the control.
Conclusion
Our study showed that the synergy of Lactobacillus postbiotics inhibited E. histolytica HM1-IMSS in vitro growth under axenic conditions, inducing morphometric alterations in trophozoites cell membrane. These results would allow designing strategies or treatments aimed at E. histolytica control in the future.
Keywords : Protozoa; Amoebiasis; Probiotic; Metabolites; Antimicrobial; Atomic force microscopy.
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