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Revista mexicana de ingeniería química

Print version ISSN 1665-2738

Rev. Mex. Ing. Quím vol.6 n.3 México Dec. 2007

 

Biotecnología

 

Producción fúngica de un pigmento rojo empleando la cepa xerofílica Penicillium purpurogenum GH–2

 

Fungal production of the red pigment using a xerophilic strain Penicillium purpurogenum GH–2

 

A. Méndez–Zavala1, J. C. Contreras–Esquivel3, F. Lara–Victoriano2, R. Rodríguez–Herrera1 y C. N. Aguilar1*

 

1 Departamento. de Investigación en Alimentos. Facultad de Ciencias Químicas. Universidad Autónoma de Coahuila. Unidad Saltillo. Blvd. V. Carranza e. Ing. José Cárdenas V. S/n, Saltillo Coah. CP. 25000., México. * Autor para la correspondencia: E–mail: cag13761@mail.uadec.mx

2 Centro Internacional de Servicios Fitosanitarios, S.A. de C.V. Urdiñola # 360. Zona Centro. Saltillo, Coah. CP. 25000, México.

3 Coyote Foods. Biopolymers and Biotechnology, S. de R. L. m i. Simón Bolivar No. 851–A. Zona Centro. Saltillo, Coah. CP.25000. México.

 

Recibido 5 de Mayo 2007
Aceptado 24 de Octubre 2007

 

Resumen

En este estudio se evaluó la capacidad del hongo Penicillium purpurogenum GH–2, de producir pigmentos tanto en medios líquidos como sólidos, así como en medios mínimos y enriquecidos. La cepa fue capaz de crecer y producir pigmentos en estos medios. Adicionalmente, se probaron 9 medios de cultivo para la producción de pigmentos, en medio sólido. El microorganismo fue capaz de crecer en todos los medios, obteniéndose diferentes velocidades de crecimiento, las mayores velocidades de crecimiento se reportaron en los medios 1, 3 y 9 (0.0903, 0.0874, y 0.0837 cm. h–1, respectivamente). La producción de pigmentos, se observó en 6 de los 9 medios analizados. De acuerdo a los barridos de exploración las máximas absorbancias se encontraron en una longitud de onda entre los 400 – 600 nm, obteniéndose 2 picos significativos de 505 y 425 nm.

Palabras clave: Penicillium purpurogenum, pigmentos, producción.

 

Abstract

In this study, the capacity of Penicillium purpurogenum GH2 for pigments production was evaluated in liquid and solid state fermentation, as well using minimum and enriched media. The strain was able to growth and produce pigments in these media. Nine culture media reported for pigment production were evaluated in solid state fermentation. Microorganism was able to of growth in all media tested, obtaining different specific growth rates, the highest specific growth rate was reported in the 1, 3 and 9 media (0.0903, 0.0874, y 0.0837 cm. h–1, respectively). The pigments production, was observed in 6 of the 9 analyzed media. In accordance with the exploration scanning analysis, the values of maximum absorbances were found between 400 and 600 nm; in this regions two peaks at 505 and 425 nm were used to evaluated the pigment concentration.

Keywords: Penicillium purpurogenum, pigments, production.

 

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Agradecimientos

Al Centro Internacional de Servicios Fitosanitarios, S.A. de C.V.

 

Referencias

Ahmed, Y.G.; Hamdy, M.E.; Mohmed, H.M. y Esam, Z.A. (2001). Production of natural microbial pigments using rice grain waste. P08–23. 11th World Congress of Food Science and Technology. Seoul, Korea.         [ Links ]

Blanc, P. J., Loret, M. O., Santerre, A. L., Pareilleux, A., Prome, D., Prome, J. C., Laussac, J. P. and Goma, G. (1994). Pigments of Monascus. Journal of Food Science 59 (4), 862–865.         [ Links ]

Blanc, P.J.; Loret, M.O. y Goma, G.A. (2001). Control of the production of metabolites by Monascus in submerged culture. Tu04–2. 11th World Congress of Food Science and Technology. Seoul, Korea.         [ Links ]

Carvalho, J. C., Pandey, A., Babitha, S. and Soccol, C. R. (2003). Production of Monascus biopigments: An overview. AgroFOOD industry hi–tech. 6, 37–42.         [ Links ]

Cho, Y. J., Hwang, H. J., Kim, S. N., Song, C. H. and Yun, J. W. (2002a). Effect of carbon source and aeration rate on broth rheology and fungal morphology during red pigment production by Paecilomyces sinclairii in a batch bioreactor. Journal of Biotechnology 000, 1–11.         [ Links ]

Cho, Y. J., Park, J. P., Hwang, H. J., Kim, S. W., Choi, J. W. and Yun, J. W. (2002b). Production of red pigment by submerged culture of Paecilomyces sinclairii. Letters of Applied Microbiology 35, 195–202.         [ Links ]

Cruz – Hernández, M., Contreras – Esquivel, J. C., Lara, F., Rodríguez, R and Aguilar, C. N. (2005). Isolation and evaluation of tannin –degrading strains from the mexican desert. Journal of Biosciences C. In press.         [ Links ]

Domínguez – Espinosa, R.M., Wang, R. and Pacho–Carrillo, J. D. (2002). Residuos agroindustriales como materia prima para la producción de compuestos químicos finos. Tecnología Ciencia Ed. (IMIQ) 17(2), 77–83.         [ Links ]

Durán, N., Teixeira, M. F. S., De Conti, R. and Espósito, E. (2002). Ecological – Friendly Pigments From Fungi. Critical Reviews in Food Science and Nutrition 42 (1), 53–66.         [ Links ]

Engstrom, G. W., Stenkamp, R. E., McDorman, D.J. and Jensen, L. H. (1982). Spectral Identification, X – ray Structure Determination, and Iron – Chelating Capability of Erythroglaucin, a Red Pigment from Aspergillus ruber. Journal of Agriculture and Food Chemistry 30, 304–307.         [ Links ]

Espinoza – Hernández, T.C. (2004). Caracterización morfológica, fisiológica y molecular de tres cepas fúngicas productoras de pigmentos. Tesis de Licenciatura. Universidad Autónoma de Coahuila. Saltillo, Coahuila, México.         [ Links ]

Fontana, J. D., Czeczuga, B., Bonfim, T. M. B., Chociai, M. B., Oliveira, Guimaraes, M. F. and Baron, M. (1996). Bioproduction of Carotenoids: The Comparative use of Raw Sugarcane Juice and Depolymerized Bagasse by Phaffia rhodozyma. Bioresources Technology 58, 121–125.         [ Links ]

Kim, C.–H., Kim, S.–W. and Hong, S.– I. (1999). An integrated fermentation – separation process for the production of red pigment by Serratia sp. KH–95. Process Biochemistry 35, 485–490.         [ Links ]

Larsen, T. O. and Breinholt, J. (1999). Dichlorodiaportin, Diaportinol, and Diaportinic Acid: Three Novel Isocoumarins from Penicillium nalgiovense. Journal of Natural Products 62, 1182–1184.         [ Links ]

Lim, G. –B., Lee, S. –Y., Lee, E. –K., Haam, S. –J. and Kim, W. –S. (2002). Separation of astaxanthin from red yeast Phaffia rhodozyma by supercritical carbon dioxide extraction. Biochemistry Engineering Journal 11, 181-187.         [ Links ]

Méndez– Zavala, A., C. N., Aguilar., Rodríguez Herrera, R. (2002). Producción de pigmentos por cepas de Penicillium sp, aisladas de la región semidesértica de México. 1er Simposium Internacional de Alimentos; Saltillo, Coahuila, México.         [ Links ]

Méndez – Zavala, A., C. N., Aguilar., Rodríguez Herrera, R. (2004). Producción de pigmentos por P. purpurogenum GH2, aislado de la región semidesértica de Coahuila, México. A–38. Memorias del IV Encuentro Nacional de Biotecnología. Santa Cruz, Tlaxcala, México.         [ Links ]

Shin, C. S., Kim, H. J., Kim, M. J. and Ju, J. Y. (1998). Morphological Change and Enhanced Pigment Production of Monascus When Cocultured with Saccharomyces cerevisiae or Aspergillus oryzae. Biotechnology and Bioengineering 59(5), 576–581.         [ Links ]

Su, Y. C. (1983). Fermentative Production of Anka–pigments (Monascus–pigments). Korean Journal of Applied Microbiology and Bioengineering 11(4), 325–337.         [ Links ]

Suhr, K. I., Haasum, I., Streenstrup, L. D. and Larsen, T. O. (2002). Factors Affecting Growth and Pigmentation of Penicillium caseifulvum. Journal of Dairy Science 85(11), 2786–2794.         [ Links ]

Wani, K. S., Naphade, B. S., Chaudhari, B. L. and Chincholkar, S. B. Pigment Production, in Concise Encyclopedia of Bioresource Technology. Ashok Pandey, PhD. The Haworth Refence Press, Inc. (2004). 645–652.         [ Links ]

Yokohama, A., Shizuri, Y. and Misawa, N. (1998). Production of New Carotenoids, Astaxanthin Glucosides, by Escherichia coli Transformants Carrying Carotenoid Biosynthetic Genes. Tetrahedron Letters 39, 3709–3712.         [ Links ]

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