Introduction
ʺPropolisʺ is etymologically derived from the Greek pro-, in defense, and polis-, the city, which means in defense of the city or hive (Ghisalberti, 1979; Bankova et al., 2019). This resinous mixture is produced by the European honey bee (Apis mellifera) from beeswax, saliva, and the exudate of various plant sources, for use primarily as a sterilizing agent and a sealant in the hive. Some tropical bees (especially Meli-pona spp.) also produce a form of propolis, and the Australian stingless bee Tetragonula carbonaria produces propolis too. The plant origins of propolis have been observed since the early 1900s, although the use of propolis dates back to at least 300 BC (Ghisalberti, 1979).
Several definitions of propolis exist; in the United States legislation (USDA, 1985), propolis is ʺa gum that is gathered by bees from various plantsʺ, while Foods Standards Australia and New Zealand (FSANZ), formerly Australia and New Zealand Food Authority (ANZFA, 2000), defines propolis as ʺa resinous substance collected by worker honey bees from the growing parts of trees and shrubs, modified by the bees and then used by the bees to seal their hiveʺ. According to the Salvadorian regulations (NSO, 2003), propolis is ʺ the product originated from resinous, gummy and balsamic substances, collected by honey bees, from buds and exudations of bark, leaves and other parts of the plants, to which the bees add salivary secretions and wax for the final elaboration of propolisʺ. Moreover, the Mexican regulations (NOM, 2017) define propolis as ʺthe resinous substance collected and processed by bees from the vegetation surrounding the apiaryʺ.
Actually, the advance on medicinal uses of propolis as an anticancer, antitumor, anti-inflammatory, cardioprotective and neuroprotective effects, has generated an increase in propolis consumption, consequently increased the requirements for its production and commercialization (ANZFA, 2000; Braakhuis, 2019; NOM, 2017). In functional food market, propolis extracts have been proposed as an ingredient against foodborne pathogens, bacteria, and disease, as well as an antifungal or oxidative stabilizer against lipid oxidation (Braakhuis, 2019; Cottica et al., 2019; Pérez et al., 2019). However, the pharmacological and preservative properties of propolis extracts can be affected by plant resin (Drescher et al., 2019), pollen (Tugba-Degirmencioglu et al., 2019), geographical origin (Letullier et al., 2020), harvest method and solvent extraction (Papotti et al., 2012), as well as seasonality and environmental conditions of the collection area (Seidel et al., 2008; de Souza et al., 2014).
Raw propolis can be classified according to its botanical origin, geographical origin, and color (NSO, 2003). In addition, in some countries there are recognized regulations that indicate the descriptors the raw propolis and their extracts must meet, such as microbial, sensory, physicochemical parameters and biological properties (NSO, 2003; NOM, 2017).
This investigation evaluated the microbial load and physicochemical parameters of raw propolis collected in two seasons and from two zones of the Sonoran Desert, and the antibacterial and antioxidant potential of propolis extracts.
Materials and methods
Chemicals and reagents
All utilized chemicals were of analytical grade. Folin- Ciocalteu’s reagent, Na2CO3, 2,2-diphenyl-1-picrylhydrazyl (DPPH), ascorbic acid (Asc), butylated hydroxytoluene (BHT), AlCl3, FeCl3, NaOH, trichloroacetic acid, NaNO2, ethanol, n-hexane, petroleum ether, plate count agar (PCA), brain heart infusion broth (BHI), and Muller-Hinton agar (MHA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid, caffeic acid phenethyl ester (CAPE), and quercetin (Qc) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Pinocembrin (Pn) was purchased from Indofine Chemical Company, Inc. (Hillsborough, NJ, USA).
Propolis collection
Propolis samples were collected from the Sonoran Desert, central Sonora, during two seasons of the year (S, summer; W, winter; 2017). The location presents desert climate characteristics, and the sampling site is surrounded by foothills or thornscrub, dominated by Fabaceae, Cactaceae, Malvaceae, and Asteraceae families (Vargas-Sánchez et al., 2016). In each season, propolis samples were collected from two collection locations: 1, Pueblo of Alamos (29°8’51.36” N, 110°7’26” W; 636 m a.s.l.); 2, Rancho Viejo (29°7’19.72”N, 110°16’58.35” W; 476 m a.s.l.), each with 15 hives.
Instrumental color evaluation
The lightness (L*), redness (a*), and yellowness (b*) of raw propolis were measured under a D65 illuminant using a spectrophotometer (CM 508d, Konica Minolta, Inc., Tokyo, Japan) with 10° observer angle. In total, ten measurements were performed on the surface of each sample (CIE, 1978).
Chemical proximate analysis
The moisture and ash contents, wax, resin, and mechanical impurities of raw propolis samples were determined according to AOAC (2005) and Lozina et al. (2010). Propolis samples (10 g) were cut into small pieces. Moisture and ash contents were gravimetrically determined by oven-drying at 100 °C for 8 h (FE-293A, Felisa, Guadalajara, Jal., Mexico) and incinerating in a muffle furnace at 550 °C for 3 h (AR-340, Felisa, Guadalajara, Jal., México), respectively. Afterward, the samples were cooled and weighed. Wax content was determined by extraction with petroleum ether at 40-60 °C for 3 h (Goldfish Fat Extractor, Labconco Corp., Kansas, MO., USA). After removing the waxes, the samples were placed in an oven at 100 °C for 3 h, cooled, and weighed. To estimate the resin contents and mechanical impurities, raw propolis samples were extracted with a mixture of n-hexane and ethanol (1:1, v/v). The insoluble residue (impurities) and residue soluble filtered (resins) were dried at 100 °C for 3 h, cooled, and weighed.
Microbiological analysis
Mesophilic and psychrotrophic bacteria counts were measured according to NOM (1994a). Each propolis sample was diluted with 0.1% of saline peptone water (1:10, w/v), and homogenized (1 min). Serial dilutions were prepared with 0.1% of peptone water, and 1 mL of appropriate dilutions were pour-plated using standard PCA. Plates were incubated at 37 °C for 48 h, and 7 °C for 10 days for mesophilic and psychrotrophic growth, respectively. Additionally, Staphylococcus aureus counts were measured according to NOM (1994b). Each appropriate dilution was pour-plated using Baird-Parker agar, spread, and incubated at 35 °C for 24 h. Counts were expressed as colony-forming units per gram of raw propolis (CFU/g).
Preparation of propolis extract
Raw propolis samples were cut into small pieces and extracted twice by maceration (300 rpm for 3 days) with ethanol (1:10, w/v) at room temperature (25 °C). The resulting solution was filtered (Whatman 4 filter paper) and concentrated under reduced pressure at 60 °C on a rotary evaporator (R-200, Büchi, Flawil, Switzerland). The obtained propolis extract was washed three times with n-hexane to remove the waxes, and resulting solution was centrifuged at 4,500 x g/4 °C for20 min (Beckman Coulter Allegra X-12, Fullerton, CA., USA) and concentrated under reduced pressure at 60 °C. The final propolis extract was lyophilized (freeze dryer model 77540, Labconco Corp., Kansas, MO., USA), and stored at −20 °C in the dark, until analysis (Hernández et al., 2007).
Total phenolic content
Total phenolic content (TPC) of propolis extracts was measured using the Folin-Ciocalteu´s method (Ainsworth & Gillespie, 2007). Each extract (100 µL, at 62.5, 125, and 250 µg of dried propolis extracts/mL of ethanol) was homogenized with 250 µL of distilled water and 250 µL of Folin-Ciocalteu´s reagent (0.25 N), and neutralized with 0.750 µL of Na2CO3 (7% w/v). The resulting solution was mixed on a vortex at 10,000 rpm for 1 min (Fisher Scientific, Pittsburgh, PA., USA), then in-cubated at 25 °C for 30 min, in the dark. The absorbance was measured using a spectrophotometer at 765 nm (Spectronic Genesys 5, Thermo Electron Corp., Madison, Wis., USA). The TPC was expressed as milligrams of gallic acid equivalents per gram of dried extract (mg GAE/g).
Total flavonoid content
Total flavonoid content (TFC) of propolis extracts was measured using the colorimetric assay described by Zhishen et al. (1999) with slight modifications. Each extract (500 µL, at 62.5, 125, and 250 µg of dried propolis extract/mL of ethanol) was homogenized with 1 mL NaNO2 (5% w/v), 1 mL AlCl3 (10% w/v), and 10 mL NaOH (1 M). The resulting solution was mixed with 12 mL of 70% ethanol and incubated at 25 °C for 15 min in the dark. The absorbance was measured at 510 nm, and results were expressed as milligrams of Qc equivalents per gram of dried extract (mg QcE/g)
Antibacterial activity
Antibacterial activity was assayed using the agar well diffusion method (Rennie et al., 2012) with slight modifications. Bacteria strains, Staphylococcus aureus ATCC 29213B, Listeria monocytogenes ATCC 7644, Escherichia coli ATCC 25922, and Salmonella typhimurium ATCC 14028 were inoculated in liquid nutrient Broth (BHI agar) and incubated at 37 °C for 24 h. Afterward, cellular suspension (1×106 CFU/mL) was grown on MHA plates by incubation at 37 °C for 24 h. Later, wells of 5 mm were punched in the agar plates using a sterile glass borer, and each extract (50 µL, at 62.5, 125, and 250 µg of dried propolis extract/mL of ethanol) was added to the wells. The plates were incubated at 37 °C for 24 h. CAPE, Pn, and Qc were used as antibacterial standards (at 250 µg/ mL). Inhibition zone (mm) around the well was recorded.
Free-radical scavenging activity
The DPPH free-radical scavenging activity (FRSA) as-say described by Molyneux (2004) was conducted, with slight modifications. Each extract (100 µL, at 62.5, 125, and 250 µg of dried propolis extract/mL of ethanol) was homogenized with 100 µL of DPPH solution (300 µM), and incubated at 25 °C for 30 min, in the dark. After, absorbance was measured at 520 nm, and the FRSA was expressed as follows: FRSA (%) = [Abs 0 - Abs s / Abs 0] × 100, where Abs 0 is control absorbance at t = 0 min, and Abs s is antioxidant absorbance at t = 30 min. In addition, Asc, BHT, CAPE, Pn, and Qc were used as antioxidant standards (at 250 µg/mL).
Reducing power ability
Reducing power ability (RPA) was measured using the Prussian blue method (Oyaizu, 1986) with slight modifications. Each extract (100 µL, at 62.5, 125, and 250 µg of dried propolis extract/mL of ethanol) was homogenized with 300 µL of phosphate buffer (0.2 M, pH 6.6) and 300 µL of potassium ferricyanide (1% w/v), and incubated in a water bath at 50 °C for 20 min. The resulting solution was mixed with 300 µL of trichloroacetic acid (10% w/v), and centrifuged (4,200 х g, 4 °C, for 10 min). The supernatant (500 µL) was homoge-nized with 100 µL of distilled water and 250 µL FeCl3 (0.1%). The absorbance was measured at 700 nm, and results were expressed as absorbance (abs) at the same wavelength.
Statistical analysis
All experiments were conducted in triplicate, with at least three independent experiments, and results were expressed as mean ± standard deviation. Data were subjected to analysis of variance, according to a two factorial design using National Center for Social Statistics Software 2007v (Kaysville, UT., USA). Normal distribution and variance homogeneity was previously tested (Shapiro-Wilk test). The season of propolis collection (summer or winter) and the location zone (Pueblo of Álamos and Rancho Viejo) were fixed terms in the model. A Tukey-Kramer multiple comparison test was performed to determine the significance of mean values at α = 0.05.
Results and discussion
Physicochemical and microbial counts of propolis samples
Raw propolis can vary on color, from red to yellow- reddish, yellow-dark, brown-green, brown, or black, depending on its geographical and plant origin (Falcão et al., 2013; NOM, 2017). The color, odor, flavor, and consistency are sensory parameters of raw propolis used for its classification in Mexico (NSO, 2003; NOM, 2017). From the color data provided in Table 1, raw propolis samples collected during winter (PW1 and PW2) showed the highest L* values (P<0.05), while a* values were similar among all samples (P>0.05). There was no seasonal effect (P>0.05) on the b* values of the samples, whereas this parameter was influenced by the collection location (P<0.05), with PW2 and PS2 registering the highest values (b* = 8.8). In agreement, Falcão et al. (2013) published the first report of propolis color evaluation in the CIELab system and indicated that this method is quick and reliable for quality recognition and could be used for propolis differentiation.
Item | PW1 | PS1 | PW2 | PS2 |
---|---|---|---|---|
Color | ||||
L* | 29.1 ± 1.0b | 27.8 ± 0.1a | 34.2 ± 0.8b | 28.9 ± 1.1a |
a* | 2.6 ± 0.3a | 2.1 ± 0.6a | 3.4 ± 0.7a | 3.5 ± 0.7a |
b* | 4.9 ± 1.0a | 3.6 ± 0.8a | 8.0 ± 1.2b | 9.6 ± 1.1b |
Proximate composition | ||||
Moisture | 4.5 ± 1.0b | 2.5± 0.4a | 4.8 ± 0.2b | 2.4 ± 0.2a |
Ash | 10.3 ± 1.1b | 6.5± 0.9a | 10.4 ± 0.5b | 7.1 ± 0.7b |
Waxes | 28.6 ± 1.9a | 27.0 ± 2.4a | 29.0 ± 2.5a | 27.0 ± 2.7a |
Resins | 34.5 ± 1.5a | 46.8± 2.0b | 35.0 ± 1.9a | 45.7 ± 1.1b |
Mechanical impurities | 21.3 ± 1.1b | 17.0 ± 1.0a | 20.3 ± 1.5b | 16.2 ± 0.5a |
Data represent mean ± standard deviation. PW1, PW2, propolis ethanol extract collected during winter from the Pueblo of Alamos and Rancho Viejo region, respectively; PS1, PS2, propolis ethanol extract collected during summer from the pueblo of Alamos and Rancho Viejo region, respectively. Different superscripts in rows indicate differences between treatments (P<0.05).
Data obtained on the proximate composition of the collected propolis samples (Table 1) revealed PS1 and PS2 samples had the lowest moisture content (P<0.05), which can be related to the weather conditions (temperature and humidity) during the collection (Vargas-Sánchez et al., 2016). In the current study, propolis samples collected during winter showed the highest (P<0.05) contents of ash and mechanical impurities (10.3 and 20.8%, respectively), which can be explained by the presence of vegetal material (leaves and woods) in the samples, insect remains, and soil, among others (NOM, 2017). Although there were no notable differences (P>0.05) in the wax content (27.4%) among the samples, propolis samples collected during summer showed the highest (P<0.05) resin content (46.2%), which is associated with the presence of phenolic compounds (Lozina et al., 2010; Bankova et al., 2019). According to NSO regulations (2003), the moisture (maximum 8%), wax (maximum 30%), and mechanical impurities (maximum 30%) are within the allowed parameters. Additionally, the results of Table 2 show no significant effect (P>0.05) between season x location region on mesophilic (<250 CFU/g), psychrotrophic (<250 CFU/g) and S. aureus (<10 CFU/g) counts. According to the NSO (2003), propolis samples showed mesophilic (<10,000 CFU/g), psychrotrophic (not established), and S. aureus counts (100 CFU/g) within the permitted limits.
Bacteria (CFU/g) | PW1 | PS1 | PW2 | PS2 |
---|---|---|---|---|
Mesophilic | <250 | <250 | <250 | <250 |
Psychrotrophic | <250 | <250 | <250 | <250 |
Staphylococcus aureus | <10 | <10 | <10 | <10 |
PW1, PW2, propolis ethanol extract collected during winter from the Pueblo of Alamos and Rancho Viejo region, respectively; PS1, PS2, propolis ethanol extract collected during summer from the pueblo of Alamos and Rancho Viejo region, respectively.
Phenolic composition of propolis extracts
Several works have reported that propolis extracts include many bioactive phenolic compounds, including phenolic acids and flavonoids (Ghisalberti, 1979; Hernández et al., 2007; Seidel et al., 2008; Tugba-Degirmencioglu et al., 2019; Papotti et al., 2012; Pérez et al., 2019). As shown in Figure 1, the results showed significant concentration-dependent differences (P<0.05) in TPC and TFC between the analyzed samples. The highest (P<0.05) TPC and TFC (>200 mg GAE/g and >300 mg QE/g at 250 µg/mL, respectively) were obtained in propolis samples collected during summer (PS1 and PS2). In agreement with our work, higher TPC and TFC were found in propolis samples collected in semiarid regions during summer than winter (Valencia et al., 2012). It has also been reported that the season of collection quantitatively affects the chemical composition of Sonoran and Brazilian propolis extracts (Valencia et al., 2012; Bueno-Silva et al., 2017). In addition, phenolic compounds, major phytochemical groups found in honey bee products, have been shown to possess antibacterial and antioxidant properties (Falcão et al., 2013; NOM, 2017).
Fig.1. Contenido total de fenoles (A) y flavonoides (B) de extractos de propóleos. Diferentes superíndices indican diferencias entre tratamientos (P<0.05). PW1, PW2, extracto etanólico de propóleos colectado durante el invierno de la región de Pueblo de Álamos y Rancho viejo, respectivamente; PV1, PV2, extracto etanólico de propóleos colectado durante el verano de la región de Pueblo de Álamos y Rancho viejo, respectivamente.
Antibacterial activity of propolis extracts
Propolis extracts and some standards were tested against foodborne pathogens (Table 3). The results showed that propolis sample × concentration had a significant effect (P<0.001) on the antibacterial activity. The concentration used was greater efficacy (P<0.05) against Gram-positive bacteria (S. aureus > L. monocytogenes) than Gram-negative bacteria (S. typhimurium > E. coli), which is consist with earlier research (Seidel et al., 2008; Nedji and Loucif-Ayad, 2014).
Treatment | (µg/mL) |
Gram-positive bacteria |
Gram-negative bacteria |
||
L. | S. | E. | S. | ||
monocytogenes | aureus | coli | typhimurium | ||
Ethanol | - | -- | -- | -- | -- |
CAPE | 250 | 8.0 ± 0.2b | 22.5 ± 0.7g | -- | 12.5 ± 0.7de |
Pn | 250 | -- | 16.0 ± 0.7d | -- | -- |
Qc | 250 | 11.0 ± 0.2c | 12.5 ± 0.1c | -- | 6.5 ± 0.7a |
PW1 | 250 | 15.5 ± 0.7e | 18.0 ± 0.1e | -- | 10.0 ± 1.4c |
125 | 8.0 ± 0.1b | 9.0 ± 0.1b | -- | 6.5 ± 0.7a | |
62.5 | -- | -- | -- | -- | |
PS1 | 250 | 12.5 ± 0.7d | 20.0 ± 1.4fg | -- | 13.5 ± 0.7e |
125 | 7.0 ± 0.1a | 9.0 ± 0.1b | -- | 8.5 ± 0.7b | |
62.5 | -- | -- | -- | -- | |
PW2 | 250 | 13.5 ± 0.7d | 17.5 ± 0.7de | -- | 9.5 ± 0.7bc |
125 | 7.0 ± 0.1a | 9.0 ± 0.1b | -- | 6.5 ± 0.7a | |
62.5 | -- | -- | -- | -- | |
PS2 | 250 | 11.5 ± 0.7c | 18.5 ± 0.7ef | -- | 11.5 ± 0.1d |
125 | 7.0 ± 0.1a | 8.0 ± 0.1a | -- | 7.0 ± 0.7a | |
62.5 | -- | -- | -- | -- |
Data represent mean ± standard deviation. CAPE, caffeic acid phenethyl ester; Pn, pinocembrin; Qc, quercetin; PW1, PW2, propolis ethanol extract collected during winter from the pueblo of Alamos and Rancho Viejo region, respectively; PS1, PS2, propolis ethanol extract collected during summer from the Pueblo of Alamos and Rancho Viejo region, respectively. (--), no in-hibition zone (0-5 mm). Different superscripts in the same column indicate differences between treatments (P<0.05).
The propolis samples had no significant effect (P>0.05) against E. coli. In addition, at 250 µg/mL the propolis samples collected during summer (PS1 and PS2) displayed greater bacterial inhibition against S. aureus and S. typhimurium than propolis samples PW1 and PW2 (P<0.05). On the contrary, no significant effect (P>0.05) on L. monocytogenes inhibition was observed between seasons. de Souza et al. (2014) noticed that propolis extracts possess antibacterial activity mainly against S. aureus, and the efficacy of this activity is affected by the season. Similarly, Chen et al. (2008) described the antibacterial activity against S. aureus, Streptococcus spp., Vibrio damsela, Bacillus cereus, and Bacillus subtilis, and was more efficacious for propolis collected during summer than winter.
NOM (2017) established that propolis extracts must exhibit a high inhibitory effect against S. aureus. In this study, the inhibition effect displayed by propolis samples against foodborne pathogens was similar to that of the antibacterial standards. Moreover, a positive and significant (P<0.05) correlation was found between the antibacterial activity, and the TPC (r 2 = 0.81) and TFC (r 2 = 0.82). It has been reported that antibacterial activity of propolis phenolic compounds can be exerted in three ways: synergistically activate some antibiotics, decrease the bacterial pathogenicity, and directly inhibit the bacterial growth. Likewise, the effectiveness depends on the polyphenols structure relationship including their hydroxyl at special sites on the aromatic rings; the methylation of the hydroxyl groups; the lypopholicity of the aromatic rings; the presence of hydrophobic substituents (including alkyl chains, alkylamino chains, prenyl groups), and nitrogen or oxygen containing heterocyclic moieties. These usually enhance the activity for propolis antioxidant compounds (Xie et al., 2015; Allawi, 2019).
Antioxidant activity of propolis extracts
The presence of phenolic compounds in honey bee products is highly correlated with the antioxidant activity (Yucel et al., 2017). As shown in Table 3, propolis extracts and some phytochemicals present in the propolis extracts were tested as antioxidants. There was a significant effect (P<0.001) of propolis sample × concentration on antioxidant activity.
As shown in Figure 2, regardless of the concentration used, the highest FRSA (P<0.05) was obtained in propolis samples collected during summer (83.2 and 85.1% of radical inhibition for PS1 and PS2, respectively). In addition, all propolis samples had a weak FRSA in comparison to the antioxidant standards (CAPE, 99%; Qc, 98%; Asc, 96%; BHT, 72%; Pn, 19%). However, at 125 and 250 µg/mL, propolis collected during winter showed a radical inhibition above 50%.
Fig.2. Actividad de eliminación de radicales libres (A) y habilidad de poder reductor (B) de extractos de propóleos. Diferentes superíndices indican diferencias entre tratamientos (P<0.05). AERL, actividad de eliminación de radicales libres; HPR, habilidad de poder reductor, Asc, ácido ascórbico; BHT, hidroxitolueno butilado; EFAC, éster fenetílico del ácido cafeico; Pn, pinocembrina; Qc, quercetina; PI1, PI2, extracto etanólico de propóleos colectado durante el invierno de la región de Pueblo de Álamos y Rancho viejo, respectivamente; PV1, PV2, extracto etanólico de propóleos colectado durante el verano de la región de Pueblo de Álamos y Rancho viejo, respectivamente.
Moreover, the season had no impact on RPA (P>0.05). The highest RPA (P<0.05) was registered in propolis samples (0.24 abs) collected in the second zone (PW2 and PS2). However, all propolis samples had a weak RPA in comparison with the antioxidant standards (Asc, 1.0 abs; CAPE, 1.0 abs; Qc, 0.89 abs; BHT, 0.75 abs; Pn, 0.35 abs). FRSA had a positive and significant (P<0.05) correlation with TPC (r 2 = 0.99) and TFC (r 2 = 0.98), while a weak correlation was found between RPA with TPC (r 2 = 0.73) and TFC (r 2 = 0.72). Consistent with these data, Chen et al. (2008) registered a higher FRSA for propolis collected during summer than winter, and a positive correlation (r 2 > 0.9) has already been found between FRSA with the TPC and TFC of propolis samples (da Silva et al., 2006).
Conclusions
In conclusion, the physicochemical parameters, phenolic composition, and biological properties of raw Sonora propolis were affected by the season and geographical region of collection. Propolis samples collected during summer presented the highest total phenolic and total flavonoids contents, as well as the highest FRSA and RPA, and displayed greater antibacterial activity against Gram-positive bacteria than Gram-negative bacteria. Therefore, propolis extracts possess promising bioactive compounds with pharmacological and preservative properties.