Abstract

PENA-LOPEZ, Jorge Luis et al. Increase of embryos somatic of Cocoa (Theobroma cacao L.) in Immersion System Automatic. Ecosistemas y recur. agropecuarios [online]. 2016, vol.3, n.8, pp.215-224. ISSN 2007-901X.

Cacao is a tropical tree native to America. Its fruit is used in the elaboration of chocolate and its derivatives. The propagation of cacao is done through sexual means or seeds; additionally, it propagates through the use of cuttings and grafting. Currently, somatic embryogenesis is also employed for the clonal propagation of cacao, which can be repeated several times and contributes to the increase in the formation of somatic embryos. Therefore, the use of a temporary immersion system can increase the production of secondary somatic embryos. Given the aforementioned, the objective was to evaluate the Rita® temporary immersion system in the performance of secondary somatic embryos from 30 to 180 days in two cultivation cycles. The results showed that somatic embryos were generated in cotyledon explants and in full somatic embryos. A second cycle was obtained from the secondary somatic embryogenesis where 150 somatic embryos were obtained per Rita® recipient with an average of 15 embryos per explant. The temporary immersion system favored the production of somatic embryos, its synchronization, and decreased the oxidation of the tissue.

Keywords : Cacao; Theobroma cacao L.; secondary somatic embryogenesis; temporal immersion..

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