Abstract

ALMARAZ-SANCHEZ, Alejandra; ALVARADO-ROSALES, Dionicio; TLAPAL-BOLANOS, Bertha  and  ESPINOZA-VICTORIA, David. Identification of fungi potentially antagonist to Phytophthora cinnamomi Rands in oak forest at the el Arrayanal, Colima and Tecoanapa, Guerrero. Rev. Chapingo ser. cienc. for. ambient [online]. 2012, vol.18, n.3, pp.341-355. ISSN 2007-4018.  https://doi.org/10.5154/r.rchscfa.2011.09.061.

The aim of this study was to identify morphologically and molecularly the mycoflora that possesses antagonistic properties on Phytophthora cinnamomi associated to the oak (Quercus spp.) soil forest at El Arrayanal, Colima, and Tecoanapa, Guerrero. Two soil samplings were done in these sites. Soil -root samples around trees with different ages and health conditions in stands affected by P. cinnamo-mi were collected. The fungi were morphologically identified by soil dilution PDA-TS (potato-dextrose-agar-tergitol-streptomycin) technique. With the obtained isolates, in vitro antagonism tests were done. Trichoderma spp, Cordyceps bassiana and Paecilomyces isolates, showed the best biological control by reducing P. cinnamomi development and showing outstanding mechanisms of space competition and mycoparasitism. The fungi were molecularly characterized by amplifying the intergenic region (ITS) of the ribosomal genes by the amplification of polymerase chain reaction (PCR) technique. For the first time C. bassiana, Cladosporium tenuissimun and six Trichoderma species associated with oak forests in Mexico are reported.

Keywords : ITS- rDNA; soil; antagonism; Quercus.

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