Abstract

SANTIAGO SANTIAGO, Dany Gabriel; VALADEZ MOCTEZUMA, Ernestina  and  CIBRIAN TOVAR, David. Molecular identification of the Botryosphaeria sp. complex related to cankers and drying of buds in Eucalyptus sp. Rev. mex. de cienc. forestales [online]. 2015, vol.6, n.32, pp.93-106. ISSN 2007-1132.

Phytosanitary problems drastically affect the production or the plants of Eucalyptus sp. from the damages that they bring. Botryosphaeria sp. is associated with cankers and drying buds, dieback, fruit rot, collar rot and damping off in seedlings, which is especially important in commercial plantations that use these species for lumber in short cycles. In order to characterize and identify at the molecular level isolates of this fungus, in the work described below is estimated, first, the genetic variability in diseased samples with genomic markers of the ISSR type, and secondly, the ITS region was sequenced and compared. The ISSR markers were analyzed with multivariate techniques to group the isolates based on their genetic similarity, of what turned out that most of the samples were very similar to each other at the genome. The results of the sequencing technique IST regions indicated the presence of Neofusicoccum sp., Neofusicoccum parvum , Diplodia corticola, Lasiodiplodia pseudotheobromae, Neofusicoccum eucalyptorum and Neofusicoccum sp. It is noteworthy that at the nucleotide level of the sequenced region, the quantity of polymorphisms among the isolates studied and those that were used as outgroups obtained from GenBank, was minimal.

Keywords : DNA; cankers; ISSR; MEGA; ITS sequence, UPGMA.

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