Abstract

FRAUSTO JAIME, Karla Andrea et al. Outbreak induction from floral stem orchid Phalaenopsis spp. (Blume) in vitro. Rev. Mex. Cienc. Agríc [online]. 2019, vol.10, n.6, pp.1207-1218.  Epub Oct 02, 2020. ISSN 2007-0934.  https://doi.org/10.29312/remexca.v10i6.608.

The induction of outbreaks in vitro is a strategy of the vegetable micropropagation used to increase the production of orchids, in comparison with the traditional crop that requires a long period of time to obtain a substantial population for commercialization. The objective of this study was to induce in vitro outbreak formation from the flower outbreaks of the orchid Phalaenopsis spp., using an organogenesis route. Four mineral salt formulations (MS 100%, MS 50%, Knudson and NPK Fertilizer 18-9-18/15-30-15) and four varieties of Phalaenopsis spp. to determine the viability of explants. Two growth regulators (6.2-benzylaminopurine BAP 20.2 µM alone and combined with naphthalenacetic acid ANA 5.37 µM) were evaluated to determine outbreak induction. The results indicate that oxidation in explants depends on the mineral salts of the culture media, as well as the variety of orchid; outbreak induction depends on the growth regulator used. BAP/ANA regulators produced 2.4 outbreaks per outbreak against 2 in BAP. The MS 50% medium proved to be the best option to maintain the viability of the flower outbreaks.

Keywords : Phalaenopsis; micropropagation; organogenesis.

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