Abstract

BERMUDEZ GUZMAN, Manuel de Jesús et al. Identifying hybrids of Citrus aurantifolia×Citrus limon using simple sequence repeats (SSR) markers. Rev. Mex. Cienc. Agríc [online]. 2017, vol.8, n.6, pp.1397-1408. ISSN 2007-0934.

Apomixis is a type of asexual reproduction where the seeds formation carries genetically identical embryos to the parent, constituting an obstacle in breeding programs for many plant species, including citrus. The identification of hybrid plants is carried out by morphological characters, isoenzymatic assays and molecular markers. The latter have been used more frequently because of their accuracy, standind out the use of random amplified polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR). In Mexican lime (C. aurantifolia) RAPD markers have only been used for hybrid identification, so there are no reports that use SSR markers for this purpose. The aim of this paper was to identify hybrids derived from controlled pollination between C. aurantifolia var. “Colimex”×C. limon var. “Rosenberg” and its reciprocal using SSR molecular markers. During the 2014-2016 years, leaves of lime trees of approximately 12 months of age were collected, which are established in the Campo Experimental Tecomán of the INIFAP. A total of eight SSR molecular markers were evaluated on the progenitors used in this study and the TAA45 and cAGG09 oligonucleotides were selected for the hybrids identification in the two progeny populations. From a total of 40 and 43 F1 individuals from the bi-directional cross between “Colimex”דRosenberg”, 17 and 35 hybrid plants were identified, respectively. The results indicate that SSR markers are efficient and reliable for the identification of Mexican lime hybrids.

Keywords : apomixis; citrus; microsatellites; molecular markers.

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