Abstract

VELASQUEZ-VALLE, Rodolfo et al. Detection of the yellow spot virus in onion seedlings in Zacatecas, Mexico. Rev. Mex. Cienc. Agríc [online]. 2017, vol.8, n.1, pp.209-214. ISSN 2007-0934.  https://doi.org/10.29312/remexca.v8i1.84.

The state of Zacatecas is one of the most important onion producing regions in Mexico. The yellow iris spot virus (IYSV) was detected in this area affecting commercial plots of this vegetable. The onion seedlings are produced in seedlings with low technology that could allow the infestation of thrips and consequently viral infection. The objective of this work was to detect the presence of the yellow spot virus in onion seedlings in Zacatecas, Mexico and to determine the correlation between the absorbance readings and the detection frequency of the IYSV. Samples of onion seedlings were obtained from 30 seedlings and a tissue sample from each seedling was analyzed by DAS-ELISA to detect the presence of the virus. No lesions were found caused by the IYSV, however, at least one seedling was positive to the virus in 90% of the seedlings sampled. The incidence of IYSV within seedlings varied between 8.3 and 100%. No clear correlation was found between the absorbance values of DAS-ELISA and the incidence of the virus.

Keywords : absorbance readings; DAS-ELISA; incidence; IYSV; varieties; thrips.

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