Abstract

ROBRES-TORRES, Emmanuel; LOPEZ-MEDINA, José  and  ROCHA-GRANADOS, Ma. del Carmen. Adventitious shoot elongation of raspberry (Rubus idaeus L.) is influenced by brassinosteroids. Rev. Mex. Cienc. Agríc [online]. 2015, vol.6, n.5, pp.991-999. ISSN 2007-0934.

In vitro culture techniques have been used to study the effect of the combination of benzyladenine (BA) and brassinolide (BL), two plant growth regulators, in the formation and adventitious shoot elongation of raspberry (Rubus idaeus L.). Rooting of regenerated shoots both in vitro and ex vitro conditions were evaluated. Three selections of raspberry, UMC-702 UMC-706 and UMC-708, generated from a breeding program at the Faculty ofAgrobiology, were selected to conduct this research. In the multiplication phase, the MS medium was supplemented with different combinations of benzyladenine (0 and 1.5 mg L^-1) and brassinolide (0, 0.00001 and 0.00002 μM). Adventitious shoot formation was not dependent on the combination of regulators; however, the elongation of it was significantly influenced by genotype and use of brassinolide (BL); the best results were obtained with UMC-706 exposed to 0.00002 μM of BL. Rooting of adventitious shoots were better in ex vitro than in vitro conditions, with higher formation and elongation both root and shoots; allowing a better acclimatization and adaptation of plants, which means savings in time and labor for micro propagation of raspberry. So, this is the first report on the use of brassinosteroids in raspberry.

Keywords : Rubus; adventitious shoots; brassinolide; shoot elongation.

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