Abstract

VELARDE FELIX, Sixto et al. Molecular and biological identification of physiological races 0 and 5 of Fusarium oxysporum Schlechtend.: Fr f. sp. ciceris (Padwick) Matuo & K. Sato of chickpea in northwest Mexico. Rev. Mex. Cienc. Agríc [online]. 2015, vol.6, n.4, pp.735-748. ISSN 2007-0934.

Fusarium oxysporum f. sp. ciceris (Foc) is a phytopathogenic fungus that causes the disease known as Fusarium wilt in chickpea cultivation. In Mexico, chickpea export only grown in the Northwest (Sinaloa, Sonora and Baja California Sur). Considering that Foc limits the production of this crop and no information concerning the identification of physiological races of this fungus in Mexico, the goal was to isolate and identify by molecular and biological tests physiological races of the fungus present in the growing areas chickpea in the northwest region of Mexico. During the period 2010-2014 were collected chickpea plants with symptoms of wilting and yellowing at different locations in the States of Sinaloa, Sonora and Baja California Sur. The fungus was isolated from small portions of the plant, which were seeded in medium potato-dextrose agar culture medium supplemented with pentanitroclorobenceno (PCNB) and chloramphenicol. We isolated and purified spore cultures, DNA was extracted for identification of physiological races by PCR and enzymatic sequencing. These strains were inoculated into chickpea in differential lines, confirming Mexico for the first time the identification of physiological races Foc 0 and 5.

Keywords : chickpea; differential lines; physiological races of Foc; sequencing.

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