Abstract

LUNA PALOMERA, C. et al. Evaluation of DNA vaccine and its recombinant peptide against inhibin alpha (165-300) on immune response and fertility in mice. Nova scientia [online]. 2018, vol.10, n.20, pp.213-221. ISSN 2007-0705.  https://doi.org/10.21640/ns.v10i20.1310.

The aim was to evaluate a DNA vaccine (INHA-DNA) and its recombinant peptide INHA-rec) against inhibin alpha (INHA) on the immune response and fertility in mice. Sixteen Balb/c mice were divided into two groups of 8 each and under a heterologous prime boost immunogenic scheme one group were inoculated with 100 μg of INHA-DNA and 14 d later with 100 μg of INHA-rec. The control group received saline solution. After the first birth, a booster was given with both antigens (INHA-DNA + INHA-rec). Immunization with 100 μg of INHA-DNA and INHA-rec induced high levels of anti-INHA antibody, as well as greater weight and size litter (P <0.05) compared to control. The mean fertility was similar between INHA (67%) and control (60%). In conclusion, the DNA vaccine and its recombinant antigen against INHA are capable of inducing immunity against INHA by increasing litter size without an apparent negative effect on fertility in mice.

Keywords : Inhibin alfa; DNA vaccine; fertility; recombinant peptide.

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