SciELO - Scientific Electronic Library Online

vol.60 issue3Application of Sodium Dodecyl Sulfate Coated Iron Oxide Magnetic Nanoparticles for the Extraction and Spectrofluorimetric Determination of Propranolol in Different Biological SamplesSynthesis and Modification of the Amyloid Peptide Sequence 37-42 of Aβ42 (AβPP): Efficient Synthesis of N-methylated Peptides, Expanding the Tools for Peptide Research author indexsubject indexsearch form
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • Have no similar articlesSimilars in SciELO


Journal of the Mexican Chemical Society

Print version ISSN 1870-249X


CRISTIANS, Sol et al. Development of a HPLC-DAD Method for the Simultaneous Quantification of 5-O-β-D-galactopyranosyl-7-methoxy-3’,4’-dihydroxy-4-phenylcoumarin and its Aglycone in Feces. J. Mex. Chem. Soc [online]. 2016, vol.60, n.3, pp.117-124. ISSN 1870-249X.

A sensitive and specific HPLC-DAD method was developed and validated for the simultaneous quantification of 5-O-β-D-galactopyranosyl-7-methoxy-3’,4’-dihydroxy-4-phenylcoumarin (4-PC) and its aglycone in rat feces. The 4-phenylcoumarins are important antidiabetic and gastroprotective bioactive metabolites of a highly commercialized medicinal plant complex in Mexico, the Copalchi complex. Both the sample preparation and the quantification method were developed; the methodology allows, for the first time ever, the simultaneous determination of the 4-PC (Rt= 2.4 min) and its metabolized aglycone (Rt= 7.5 min), aimed to their pharmacokinetic analysis, specially their elimination. Linearity was determined in the range, 0.09-4.5 µg/mL for both compounds (R2= 0.9999). Accuracy was <15.0% for 4-PC and aglycone with an inter-assay precision of maximum %RSD of 6.1% for 4-PC and 2.7% for the aglycone. The intra-assay precision was %RSD < 7.5 for 4-PC and < 3.5% for the aglycone. Analyte recovery from spiked samples was always > 98.02% for 4-PC and > 96.61% for its aglycone. The method was successfully applied to a single-dose preclinical pharmacokinetics preliminary study in rats.

Keywords : 4-phenylcoumarin; aglycone; Copalchi; HPLC-DAD; pharmacokinetic.

        · abstract in Spanish     · text in English     · English ( pdf )