Abstract

RADECKI, Jerzy  and  RADECKA, Hanna. Mechanisms of Analytical Signals Generated by Electrochemical Genosensors: Review. J. Mex. Chem. Soc [online]. 2015, vol.59, n.4, pp.276-281. ISSN 1870-249X.

Detection and analysis of specific DNA sequences is an important approach in molecular diagnosis. Avian influenza viruses (AIVs), in particular the highly pathogenic H5 subtype, could cause severe diseases. They are endemic in wild birds and their introduction and conversion to highly pathogenic avian influenza virus in domestic poultry is a cause of serious economic losses as well as a risk for potential transmission to humans. We report a short review of electrochemical genosensors devoted for detection of influenza virus H5N1 gene sequence. We will focus our attention on ion-channel mechanism, E-DNA sensors and genosensors based on redox active layer. A novel a dual DNA electrochemical sensor with "signal-off" and "signal-on" architecture for simultaneous detection of two different sequences of DNA derived from Avian Influenza Virus type H5N1 by means of one electrode is presented.

Keywords : electrochemical genosensors; electrode modification; redox active monolayers; mechanisms of analytical signals generation.

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