Abstract

CANIZARES-MACIAS, María del Pilar; GONZALEZ-SAN MIGUEL, Hilda María  and  CERDA, Víctor. Implementing Stepwise Solvent Elution in Multisyringe Liquid Chromatography (MSC) to Separate Norepinephrine, Epinephrine and Dopamine. J. Mex. Chem. Soc [online]. 2012, vol.56, n.2, pp.194-200. ISSN 1870-249X.

A multisyringe liquid chromatographic (MSC) method by using stepwise solvent elution and reverse-phase liquid chromatography C₁₈ monolithic column to separate Epinephrine (E), Norepinephrine (NE) and Dopamine (DA) was developed. The first step employs the mixture sodium1-hexanosulfonate monohydrate (HS) 7.5mM: methanol (98:2) to separate NE and E and the second step uses HS (5.0 mM): methanol (92:8) to elute DA. The three analytes were separated on 6 min spending only 10 mL of mobile phases. The detection limits were between 1.3 μg mL^-1 (DA) and 2.04 μg mL^-1 (NE).

Keywords : Multisyringe liquid chromatography; C₁₈ monolithic column; catecholamines; stepwise elution.

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