Abstract

NAVARRO-GARCIA, Víctor M.; HERRERA-RUIZ, Maribel; ROJAS, Gabriela  and  ZEPEDA, L. Gerardo. Coumarin Derivatives from Loeselia mexicana: Determination of the Anxiolytic Effect of Daphnoretin on Elevated Plus-maze. J. Mex. Chem. Soc [online]. 2007, vol.51, n.4, pp.193-197. ISSN 1870-249X.

The bis-coumarin daphnoretin (1) and its monomelic precursors scopoletin (3) and umbelliferone (4) were isolated for the first time from L. mexicana, a vegetal species used in Mexican traditional medicine. The unambiguous ¹³C NMR chemical shifts assignments of daphnoretin (1) and its acetyl derivative 2 were achieved through one-bond (gHMQC) and long-distance (gHMBC) heteronuclear correlations. The crude hydroalcoholic extract and pure daphnoretin 1 obtained from this plant, showed anxiolytic effect in male ICR mice exposed to elevated plus-maze. Different doses of daphnoretin (1) were not able to modify the spontaneous locomotor activity measured in the open field test. This is the first report regarding to the anxio-lytic effect of daphnoretin (1).

Keywords : Loeselia mexicana Brand; daphnoretin; 7-acetyldaphnoretin; ¹³C NMR chemical shift; elevated plus maze; anxiety.

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