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Revista odontológica mexicana

Print version ISSN 1870-199X

Abstract

TINOCO CABRIALES, VC et al. Leukocyte death incited by propolis toxicity. Rev. Odont. Mex [online]. 2013, vol.17, n.3, pp.161-165. ISSN 1870-199X.

Objective: The aim of the present study was to assess the extent of propolis' in vitro cytotoxicity on polymorphonuclear leukocytes. Materials and methods: The present study was an in vitro, controlled, experimental endeavor. Statistical procedure entailed variance analysis of repeated measures as well as Scheffe's post-hoc. A p < 0.05 statistical significance was established. To obtain leukocytes, 10 mL of peripheral venous blood was harvested from six randomly selected, healthy, 20-30 year old subjects, of both genders. Results: Scheffe's analysis with 95% reliability for comparison between control and experimental groups. Significant with p 0.0001 for propolis 1 control 1 and propolis 2 control 2. For propolis 1 propolis 2 p 0.5002/control 1 control 2 p 0.9621. Conclusions: In the present experiment propolis at a 1:4 dilution applied for 1-2 hours to polymorphonuclear leukocytes caused 70% cellular death. This resulted in statistical significance.

Keywords : Cytotoxicity; propolis; neutrophil polymorphonuclear leukocytes.

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