Abstract

OROZCO-SANCHEZ, F. et al. Oxygen limitations to grow Azadirachta indica cell culture in shake flasks. Rev. Mex. Ing. Quím [online]. 2011, vol.10, n.3, pp.343-352. ISSN 1665-2738.

It was evaluated the growth of Azadirachta indica cell suspension in different conditions of oxygen delivery in Erlenmeyer shake flask. Oxygen transfer rate (OTR, kg O₂ m^-3 day^-1) for the closures utilized were: silicone foam (1.04), cotton (0.58), and aluminum foil (0.07). A. indica cells growing during 6 weeks of subculture showed that lower OTR reduced cell viability, the pH of broth medium, and Azadirachtins production. While, higher OTR induced the formation of aggregates. Using a stirred tank bioreactor, it was determined that A. indica cells had an oxygen consumption of 0.100 kg O₂ kg DW^-1 day^-1, a higher value than other plant cell cultures. These results show that OTR generated in Erlenmeyer s hake flasks is lower to oxygen uptake rate of A. indica cells and it is a limiting factor to grow this plant.

Keywords : Azadirachta indica cell culture; shake flask; oxygen transfer rate; oxygen transfer resistance; closures.

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