Abstract

ESPINOSA-AYALA, G.E. et al. Cell culture media desing for mammalian cells by using alternative sources of nitrogen and vitamins. Rev. Mex. Ing. Quím [online]. 2007, vol.6, n.3, pp.243-246. ISSN 1665-2738.

Culture media formulation has a direct influence on growth, morphology and physiology of cells. In this work, a mammalian cell culture medium was formulated based on peptone and yeast extract with an industrial reagent level. Three different mammalian cell lines, OK, CHANG y LLCPK-1 were successfully grown in a 25:75 mix of MEM:MD (MEM = control medium; MD = house medium) as determined by comparing cell growth parameters, cellular morphology and susceptibility to HgCl₂ with those obtained from cells grown in MEM medium. A greater amount of MD in the mix 5:95 produced nuclear morphological changes, altered nucleus-cytoplasm relationship and did not support cell growth, which was possibly due to a toxic concentration of metals in the peptone and yeast extract and also to the low concentration of zinc in the yeast extract.

Keywords : cell culture medium; cell adaptation; peptone; yeas extract.

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