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Biotecnia

On-line version ISSN 1665-1456

Abstract

AGUILAR-USCANGA, Blanca Rosa et al. Staphylococcus aureus inactivation and maintenance of macronutrients of human milk by high hydrostatic pressure and spray-drying process. Biotecnia [online]. 2023, vol.25, n.2, pp.105-112.  Epub Aug 25, 2023. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v25i2.1872.

Human milk banks (HMB) use pasteurization and freezing methods to preserve the milk, nevertheless, the nutritional value of the human milk (HM) decreases during thawing and storage. This study aimed to evaluate the effect of high hydrostatic pressures (HHP) and spray-drying on macronutrients, microbiological quality, and inactivation of Staphylococcus aureus on HM. The characterization of HM powder, reduction of S. aureus, and modifications in microbiological viability, proteins, carbohydrates, lipids, and ashes were assessed. The findings demonstrated that while S. aureus and all other examined microbial groups were undetectable, macronutrient concentrations remained constant during the entire conservation process. High yield (> 99 %) was achieved thanks to the inclusion of soluble fiber during the drying process, and the produced powder displayed high solubility, low moisture content, and activity water; which are desirable properties in dried foods. Therefore, the combination of HHP and the spray-drying process is an alternative to facilitate handling, improve the microbial quality, allow the addition of oligosaccharides, and maintain the nutritional value of HM in HMB.

Keywords : Human milk; high hydrostatic pressure; spray drying; Staphylococcus aureus; milk preservation.

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