Abstract

RAMIREZ MENDEZ, Jesús Eduardo et al. Biological effectiveness of Agave striata and Fouquieria splendens extracts against Clavibacter michiganensis subsp. michiganensis. Biotecnia [online]. 2023, vol.25, n.1, pp.34-42.  Epub Aug 07, 2023. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v25i1.1751.

Bacterial canker is one of the tomatoes (Solanum lycopersicum) diseases caused by the bacterium Clavibacter michiganensis subsp. michiganensis (Cmm). In this study, Cmm was identified by end-point PCR using the primers CMM5F and CMM6R. The efficacy of methanolic extracts of Agave striata and Fouquieria splendens on Cmm in vitro under greenhouse conditions was evaluated, as well as information about the composition of phytochemicals present in plants. The in vitro assay was performed using the plate microdilution technique from 3.9 mg/L to 1000 mg/L, the Ci50 and Ci90 of each extract was determined. In the greenhouse experiment, the effect of the extracts and an organic product “Biobacter” against Cmm was evaluated on incidence, severity, and morphometric parameters. The in vitro results indicated that the extracts of F. splendens and A. striata showed inhibition on the phytopathogen. The greenhouse trial showed that the incidence, severity and morphometric parameters were lower with F. splendens extract, subsequently, with Biobacter product.

Keywords : Clavibacter michiganensis subsp. michiganensis; tomato; methanolic extracts; biological effectiveness.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )