Abstract

CARRANZA-ALVAREZ, Candy et al. Effect of natural extracts on the micropropagation of Vanilla planifolia Jacks. ex Andrews (Orchidaceae). Biotecnia [online]. 2021, vol.23, n.1, pp.5-12.  Epub June 18, 2021. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v23i1.805.

Vanilla planifolia Jacks. ex Andrews is an orchid of great economic importance, which faces problems due to overexploitation and threat of its original habitat. Therefore, the aim of the present investigation was to establish an in vitro micropropagation protocol evaluating the effect of different organic extracts.

The first stage of the in vitro culture was the seeds germination leading to the formation of protocorms. The protocorms were grown in MS media supplemented with natural extracts: MPL (banana), MPI (pineapple), MCO (coconut water) and control medium, to promote morphogenesis. Subsequently, to induce shoots rhizogenesis and acclimatization, auxin treatments (AIA and AIB) were used. The results showed that in vitro germination began at 60 days, and that the addition of organic extracts promoted the differentiation of the shoots, resulting the MPI treatment more efficient. Furthermore, the addition of 0.5 mg L-1 of IAA promoted vitroplants rooting and pre-acclimatization. These results suggest that organic extracts promote the vanilla morphogenesis, without synthetic growth regulators in the early stages of in vitro micropropagation.

Keywords : vanilla; micropropagation; natural extracts.

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