Abstract

MONTOYA-RODRIGUEZ, Alvaro et al. Evaluation of the in vitro and in vivo antihypertensive effect and antioxidant activity of blue corn hydrolysates derived from wet-milling. Biotecnia [online]. 2020, vol.22, n.2, pp.155-162.  Epub Aug 07, 2020. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v22i2.1257.

Hypertension is considered a risk factor for coronary heart disease, and its prevalence has increased substantially. Inhibition of angiotensin-converting enzyme (ACE-I) is key to lower blood pressure, making it an excellent treatment for hypertension. Corn (Zea mays L.) is an important source of bioactive peptides with potential anti-hypertensive activity related to ACE-I inhibition. These peptides can be obtained through the hydrolysis of corn gluten meal (CGM), as wet-milling by-products. The aim was to evaluate the in vitro and in vivo ACE-I inhibitory activity of blue CGM hydrolysates. Enzymatic digestion in vitro of blue CGM was conducted at different times. Hydrolysis for 360 min significantly increased both soluble protein and antioxidant activity by 4 and 8-fold respectively, the maximum ACE-I inhibition (94.3 %) was observed with 260 min hydrolysate. Mice were treated with the blue CGM hydrolysate (260 min), captopril or PBS to test the bioavailability in vivo. The CGM hydrolysate was detected in serum after 5 and up to 30 min after ingestion, showing the maximum ACE-I inhibitory capacity (59 %) during the first 15 min. Overall, this work showed that the blue CGM hydrolysate could serve as a functional food ingredient with antihypertensive effect due to its blood pressure-lowering peptides.

Keywords : blue corn; wet-milling; corn gluten meal; antihypertensive; antioxidant activity.

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