Abstract

HIPOLITO-ROMERO, Enrique et al. Brief approach to the genomic nature of Moniliophthora roreri CPMRT01 isolated from cocoa in Tabasco, Mexico. Biotecnia [online]. 2020, vol.22, n.2, pp.39-49.  Epub Aug 07, 2020. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v22i2.1244.

Moniliophthora roreri is a fungus of extreme agroeconomic importance mainly in the American continent, since it is the causal agent of moniliasis of at least four cocoa species. The genome of this fungus consists of approximately 52,3 Mbp, whose genes of interest may be regrouped depending on their nature (e.g. hemibiotrophics, biotrophics and phytopathogenics). On the other hand, Moniliophthora is liable to metabolize proteins involved in infection processes, metabolic regulation and defense mechanisms. The aim of this work was to partially characterize a fragment of the genome of a M. roreri strain previously isolated from cocoa in the State of Tabasco, Mexico. To reach this goal, an experimental strategy was implemented that involved the generation of genomic libraries as well as a consensus nucleotide region sequencing, functional-heuristic prediction and identification of hypothetical conserved domains, respectively. The study allowed to explore about 16 % of the strain´s genome. Among the most interesting results and conclusions obtained, phylogenetic reconstructions of maximum identity respect to the reference genome were reached, whose transcripts would be involved in phytopathogenic processes and secondary metabolism, identifying apparent protein domains and active catalytic sites in some of the contigs under study.

Keywords : genome; moniliasis; phylogenetic reconstruction, phytopathogenic basidiomycete; secondary metabolism.

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