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On-line version ISSN 2521-9766Print version ISSN 1405-3195


HERRERA-PEREZ, Jerónimo et al. In vitro ruminal fermentation and emission of gases of diets with different inclusion of sunflower seed (Helianthus annuus). Agrociencia [online]. 2018, vol.52, n.8, pp.1071-1080. ISSN 2521-9766.

Enteric methane (CH4) is produced during the process of energetic fermentation and represents an energy loss of 2 to 15 %. The seeds of oleaginous plants in the feed of ruminants are an alternative for reducing the production of CH4. Therefore, the objective of the present study was to determine in vitro the production of CH4, carbon dioxide (CO2) and the fermentative characteristics in diets for lambs with different levels of sunflower seed. Treatments were 0 (T1), 6 (T2), 12 (T3) and 18 % (T4) of inclusion of sunflower seed in the base diet. The diets were evaluated for production of CH4, CO2, production of volatile fatty acids (VFA), degradation of dry matter (DEGDM), neutral detergent fiber (DEGNDF) and acid detergent fiber (DEGADF), as well as the total bacteria count (TB) at 72 h of incubation. The experimental design was completely randomized, and an analysis of orthogonal polynomials was made to evaluate the linear and quadratic effects of the treatments. When the content of sunflower seed in the diet was increased, there was a reduction of DEGDM, DEGNDF and DEGADF (p≤0.05). The content of VFA after 72 h of fermentation showed a linear reduction (p≤0.05) when the content of sunflower seed was increased. The production of CH4, CO2 and TB count did not present differences among treatments (p>0.05). Therefore, increasing the amount of sunflower seed in the diet reduced the degradation capacity of its components.

Keywords : gas production; methane; in vitro; degradation of dry matter; sunflower seed.

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