Abstract

LEY-DE COSS, Alejandro et al. Rumen bacteria growth in a culture medium of Jatropha curcas L. pulp without detoxification. Agrociencia [online]. 2016, vol.50, n.8, pp.1001-1011. ISSN 2521-9766.

The pulp of Jatropha curcas L. contains up to 60 % of crude protein; still, it is not used as ruminant feed due to its content of phorbol esters and other toxic compounds. These compounds have been extracted and quantified with chemical, physical and biological methods with variable outcomes. Furthermore, studies related to the use of this substrate by rumen bacteria are limited. The objectiveof this study was evaluate the fermentation activity of a bacteria consortium selected in a J. curcas culture pulp medium, without detoxification. As substrate, the culture media was prepared with 0.2 g of J. curcas pulp, inoculated with fresh rumen fluid (FRF) and incubated at 38.5 °C for 10 d. The treatments were (sources of inoculum): T1, fresh ruminal fluid (FRF); and T2, rumen bacteria utilizing J. curcas pulp (BRUpj). The experimental design was completely randomized with four replications. The variables were: total bacterial concentration, volatile fatty acids, ammonium nitrogen, pH of the medium and dry matter in vitro degradation (DMivD) at 3, 6, 12, 24, 48, 72 and 96 h of incubation. In the total bacterial population the DMivD and pH between treatments showed no statistical difference (p>0.05). Acetic acid concentration was higher in T1 (p(0.05) at 3, 6 and 12 h, and NH4 was higher (p≤0.05) in T2 at all times. Bacteria in FRF and in BRUpj showed ability to degrade the J. curcas pulp without detoxification.

Keywords : Ruminants; rumen microbiology; Jatropha curcas.

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