Abstract

MEJIA-FRANCO, Rafael; ARZATE-FERNANDEZ, Amaury M.; JARAMILLO-FRIAS, Alejandro  and  VAZQUEZ-GARCIA, Luis M.. Differential expression of biochemical markers during vegetative development and reproductive stages of the aztec lily (Sprekelia formosissima (L.) Herbert). Agrociencia [online]. 2016, vol.50, n.8, pp.989-999. ISSN 2521-9766.

Some isozymes are involved in processes of interest to geneticists, such as flowering, plant development, height, pest and disease resistance, to name some. Therefore, it is important to determine, using biochemical markers, if there is differential expression during the vegetative and reproductive phases in the Aztec lily (Sprekelia formosissima (L.) Herbert). To do so, during those stages we collected tissue from the leaves, tepals gynoecium and stamens. These were then macerated, an extraction buffer was added and the total liquid extracts were used to determine isozymatic patterns via electrophoresis in starch gels. The differential enzymatic activity was evaluated using eleven isozymatic systems, from which only nine enzyme forms or isozymatic band patterns (IBP) were observed: two bands with peroxidase (POX; EC 1.11.1.7), three bands with acid phosphatase (ACP; EC 13.1.3.2), one with phosphoglucose isomerase (PGI; EC 5.3.1.9), two for phosphoglucomutase (PGM; EC 2.7.5.1) and one for malate dehydrogenase (MDH, EC 1.1.1.37), which showed the same pattern of isozymatic banding at all stages of the plant development. For the purpose of this research we only considered here the isoenzymatic banding patterns that were different.

Keywords : Sprekelia formosissima; isozymes; differential expression.

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