Abstract

CERNA, Ernesto; OCHOA, Yisa; BADII, Mohammad  and  LANDEROS, Jerónimo. Adaptation of methods using microplates to identify mechanisms of resistance in Tetranychus urticae Koch. Agrociencia [online]. 2010, vol.44, n.6, pp.623-630. ISSN 2521-9766.

Biochemical tests are useful to learn about the activity and amount of enzymes responsible for resistance. In this study we collected mites Tetranychus urticae Koch in strawberry production fields in the state of Guanajuato, Mexico. Biochemical tests were performed to determine the enzymatic mechanisms of resistance, using a susceptible line of T. urticae as a reference, and adapting the methods in microplates, as described by Fersht for mosquitoes. Once the method was standardized, the level of enzymes in both populations was determined. It was found that oxidases and α y β esterases have greater presence in the field population, hence they are responsible for the resistance to acaricides by the population of T. urticae sampled in this study.

Keywords : tests in microplates; esterases; oxidases.

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