Abstract

CASTRO GARIBAY, Sandra Luz; VILLEGAS MONTER, Angel  and  CRUZ LARIOS, Iris Jacaranda. Kinetin concentration and explant type in in vitro multiplication of Sequoia sempervirens (D. Don). Endl. Polibotánica [online]. 2023, n.55, pp.81-94.  Epub May 26, 2023. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.55.6.

Sequoia sempervirens is the tallest tree in the world, it has a low germination percentage, due to it the in vitro tissue culture has been used for its propagation. Even so, the multiplication technique development is still not efficient. This work aimed to the development of an efficient protocol for in vitro multiplication of S. sempervirens, using apical and basal stem explants, and four concentrations of kinetin and a culture medium with a low concentration of mineral salts to replace the use of the MS medium. Twenty axillary buds from an 18-years-old tree were established in vitro, after three subcultures, three experiments were established every six weeks. Kinetin (6- furfuryl- aminopurin) concentrations (0, 0.92, 1.85 y 3.71 µM) and two types of explants: apical and basal, were evaluated. The parameters evaluated were: height increase, over time growth, and number of axillary shoots emitted by explant type. The height increase for the three experiments was in apical explants (2.35, 4.26, and 4.78 cm) with 0 µM kinetin. The greatest increases in height of apical explants (2.35, 4.26 and 4.78 cm) were obtained with 0 μM of kinetin, in the three experiments. The greatest increases in height were in all evaluated treatments in the second and the fourth week. The greatest number of axillary shoots (2.11) were obtained in basal explants. The in vitro propagation protocol for S. sempervirens using apical and basal explants is efficient, the growth of the explants was successful with 12.38 mm of total nitrogen in culture medium.

Keywords : Sprouting; cytokinin concentrations; micropropagation; vegetative propagation.

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