Abstract

LOPEZ PUC, G.  and  HERRERA-COOL, G.J.. Asymbiotic germination, in vitro conservation and regeneration of Catasetum integerrimum. Polibotánica [online]. 2022, n.53, pp.135-149.  Epub Apr 04, 2022. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.53.9.

The deforestation and illegal over-collection of orchids have generated the need to propose alternatives to conserve and multiply them. One orchid of interest is Catasetum integerrimum, whose habitat is in Central America and Mexico. This study aimed to provide methods for the conservation and sustainable use of C. integerrimum. Asymbiotic seed germination was established by evaluating four formulations of basal media: 1) Murashige and Skoog (MS) with macro and micronutrients and vitamins; 2) MS with macro and micronutrients without vitamins; 3) Basal medium for orchids with activated carbon and banana powder; 4) Knudson basal medium. We developed the slow-growth protocol for which evaluated MS basal medium concentration (full and half of the ionic strength) and the effect of sorbitol, mannitol, and sucrose at 1, 2, and 3%. The influence of three explant types (root, node, and leaf) and kinetin at 4.64, 9.29, and 23.2 µM and Benzyl Amino Purine (BAP) at 4.43, 8.87, and 22.2 µM were evaluated to establish in vitro regeneration for plant multiplication. Asymbiotic germination of C. integerrimum was successful on Murashige and Skoog medium with macro and micronutrients without vitamins. In vitro conservation was viable in half-strength basal MS medium supplemented with 2% sorbitol; it was the best treatment for conserving seedlings under slow-growth conditions after six months of in vitro culture. Node explant formed direct shoot organogenesis in MS with BAP or kinetin at all concentrations evaluated. Indirect shoot organogenesis was observed in root explant in MS supplemented with 4.64 or 9.29 µM kinetin.

Keywords : slow growth; kinetin, mannitol; sorbitol, medium-term conservation.

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