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Polibotánica
Print version ISSN 1405-2768
Abstract
RUIZ-SALAZAR, R. et al. Population structure analysis of ayocote beans (Phaseolus coccineus L.) By AFLP. Polibotánica [online]. 2019, n.47, pp.13-24. ISSN 1405-2768. https://doi.org/10.18387/polibotanica.47.2.
Ayocote beans (Phaseolus coccineus L., 2n=2x=22) are a legume native from Mexican highlands; this is one species that has been poorly studied, despite its importance for human feeding and its potential for Phaseolus breeding of disease resistance as well as against some abiotic factors as cold tolerance. The aim of this study was to determine the population structure of 117 collections of P. coccineus from 15 locations of the Huasteco Karst from Puebla, Mexico, plus five controls in order to to know the degree of genetic variability present in Mexican native germplasm materials of P. coccineus by using the Amplified Fragment Length Polymorphism (AFLP) molecular marker strategy. The optimum genetic structure indicated a delta K value = 3. The polymorphism was 87.4% and the AMOVA confirmed the low genetic differentiation among accessions (Fst = 0.067), the genetic variance explained among and within populations was 6.7 and 93.3%, respectively. Cluster analysis did not show groupings based on germplasm origins.
Keywords : Phaseolus coccineus; populations; accessions; diversity.