Abstract

TORRES-GARCIA, B.E.; MORALES-DOMINGUEZ, J.F.; FRAIRE-VELAZQUEZ, S.  and  PEREZ-MOLPHE-BALCH, E.. Obtention of transformed roots cultures of the medicinal plant Bidens odorata Cav (Compositae) and preliminary phytochemical analysis. Polibotánica [online]. 2018, n.46, pp.241-257. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.46.16.

An in vitro propagation protocol for Bidens odorata Cav. was developed by culturing nodal stem segments on Murashige and Skoog medium added with cytokinins. The best results were 5.5 ± 0.4 shoots per explant with benzyladenine, and 4.9 ± 0.3 with N6-(2-isopentenyl)-adenine. Their susceptibility to genetic transformation with three strains of Agrobacterium rhizogenes was also evaluated. The best was the A4/pESC4 strain, at a bacterial density of 107 CFU mL-1, which generated stable and active growth transformed roots cultures with a biomass accumulation of 4.8 times the initial inoculum at the third week of culture. The verification of the transformation was made histochemically by the GUS test, being positive from 7 to 82% of the evaluated roots; As well as molecularly, by amplifying the fragments corresponding to the rolB, gus and nptII genes. Phytochemical screening performed by thin layer chromatography revealed the ability of transformed roots to synthesize compounds of various types, suggesting the presence of alkaloids, anthracene derivatives, essential oils, flavonoids, saponins, steroids and triterpenes. These are present in the aerial part or roots of B. odorata, so that these results showed that the transformed roots may be a viable option for obtaining the compounds of pharmacological interest of this species.

Keywords : Agrobacterium rhizogenes; secondary metabolites; TLC; transformed roots.

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