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Madera y bosques
On-line version ISSN 2448-7597Print version ISSN 1405-0471
Abstract
VELASQUEZ, Jesús; TORO, María Elena; ROJAS, Luis and ENCINAS, Osvaldo. Actividad antifúngica in vitro de los extractivos naturales de especies latifoliadas de la Guayana Venezolana. Madera bosques [online]. 2006, vol.12, n.1, pp.51-61. ISSN 2448-7597. https://doi.org/10.21829/myb.2006.1211250.
The microbiological effect of the secondary metabolites developed as mechanism of passive defense, in the wood of the species Puy (Tabebuia serratifolia (Vahl.) Nicholson), Zapatero (Peltogyne porphyrocardia Griseb.), Algarrobo (Hymenaea courbaril L.) and Cartan (Centrolubium paraense Tul. var. orinocense Benth) was evaluated under laboratory conditions, against two common microorganisms that cause deterioration on the wood G. traveum and T. versicolor. Antifungal assay was carried out using the agar dilution method with superficial inoculation. Solvents, like acetone, ethanol and water, of different polarity were evaluated for the recovery of the extractives. Malt extract agar was used in the antifungal assay at concentration of 0,05%, 0,1% and 0,2% (v/v). The growth inhibition was used as a measure to evaluate the effectiveness of the natural chemical product. Results of the preliminary evaluation demonstrated that all the evaluated extracts present biological activity associated with the microorganisms test. The antifungal activity of the soluble extract in water, sustains that it is the most tolerant for the microorganisms that deteriorate wood. This may be attributed to the presence of organic materials called heartwood´s sugar. The acetonic and ethanolic extracts showed to have the capacity to extract the higher quantity of active biologically compound of the heartwood of the species, producing higher growth inhibition of the microorganisms used in the study.
Keywords : Antifungal activity; heartwood; extractives; secondary metabolites; natural products.