Abstract

LEE-ESPINOSA, H. E. et al. Somatic embryoids encapsulation of Laelia anceps ssp. dawsonii to produce synthetic seed. Rev. Chapingo Ser.Hortic [online]. 2009, vol.15, n.spe, pp.33-40. ISSN 2007-4034.

Somatic embryoids obtained from germinated seeds in an MS culture media supplemented with naphthalene acetic acid (NAA), 6, bencylaminopurine (BAP) and indole-3-acetic acid (IAA), 2 mg·liter^-1 each, were encapsulated in sodium alginate in complex with calcium chloride (CaCl₂·2H₂O) 75 mM matrix, to produce synthetic seeds, as a rescue and conservation strategy. In order to determinate the effect of physical and chemical factors, such as stored temperature and time, sodium alginate consistency, and mineral nutriments an plant growth regulators concentration on percentage and time for germination, and the viability of the synthetic seeds, different concentrations of: sodium alginate (2.0, 3.0 and 4.0%), MS salts (37.5, 50 and 100%) and BAP (0.5, 1.0 and 2.0 mg·liter^-1) added to the encapsulation matrix, were tested at different temperatures (4, 20 and 25 °C) and incubation times (15 and 30 days). A hundred percent germination of synthetic seeds was obtained when 3% sodium alginate in complex with CaCl₂·2H₂O at 75 mM was used; MS salts at 100 and 50% and 2.0 mg·liter^-1 BAP supplemented to the encapsulation matrix, achieved 64.54 y 62.77% germination rate, respectively. No meaningful difference was observed between concentrations.

Keywords : Orchidaceae; artificial seed; somatic embryogenesis.

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