Abstract

ANGELES CANAS, Adriana Noemí  and  CANIZARES MACIAS, Ma. del Pilar. Desarrollo de un sistema sensor para la cuantificación de glucosa en jugos de frutas. Rev. Soc. Quím. Méx [online]. 2004, vol.48, n.1, pp.106-110. ISSN 0583-7693.

A sensor system for the determination of glucose in fruit juices using an enzymatic reactor packed with the immobilized glucose oxidase enzyme is proposed. The determination is based in a series of reactions, which area carried out sequentially in different places of the manifold: 1) oxidation of the glucose, by means of immobilized glucose oxidase, to form H₂O₂, 2) reaction between H₂O₂ and the iodide ion to form iodine and 3) development of the triiodide which is measured at 353 nm. The lineal range was 0.038-6.66 mM, detection limit 0.014 mM and the precision 1.4 %. The method was applied to fruit juices where the pretreatment of the samples was a 1:100 dilution and the addition of KIO₃ to eliminate the ascorbic acid interference.

Keywords : Sensor system; glucose; immobilized glucose oxidase; fruit juices.

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