Abstract

MARQUEZ ROCHA, Facundo J.. Producción de vitamina B₁₂ por Methanosarcina sp. utilizando metanol y acetato como fuente de carbono. Rev. Soc. Quím. Méx [online]. 2000, vol.44, n.3, pp.209-214. ISSN 0583-7693.

Two strains of the genus Methanosarcina were isolated, both with the ability to utilize simultaneously acetate and methanol as carbon source. The maximum production rate of CH₄ was 1.43 (strain C1) and 1.30 mmol h⁻¹ (strain C2), under a concentration of 100 and 200 mM acetate, respectively. Strain C2 shown better resistance to high acetate concentration (> 150 mM). The yields of CH₄ were 0.45 and 0.46 mmol_CH4 mmolM_OH⁻¹, for the strains C1 and C2, respectively. The higher extra cellular production of vitamin B₁₂ was achieved to an acetate concentration of 50-150 mM in both strains, but vitamin B12 amount was 1.8 times in C2 (9 mg L⁻¹h⁻¹) to that produced by C1 (5 mg L⁻¹ h⁻¹).

Keywords : Vitamin B₁₂; Methanosarcina; carbon source.

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