Abstract

JUAREZ ESTRADA, Marco Antonio; MOLINA HERNANDEZ, Jessica Alejandra  and  GONZALEZ SOTO, Lourdes. Enhancement of competitive exclusion by a defined probiotic on Salmonella enterica serovar Enteritidis colonization during rearing of Leghorn chicks. Vet. Méx [online]. 2010, vol.41, n.1, pp.25-43. ISSN 0301-5092.

Competitive exclusion degree from a defined (DP) and undefined probiotic (UDP) administered to one-day Leghorn chicks and challenged with 1 x 10⁸ CFU of Salmonella enterica serovar Enteritidis fagotype 13^A (SE) was evaluated. Birds with DP at 20 day old showed 21.7% of SE positive isolates in liver-spleen (LS), less than 51.7% recorded from birds without any probiotic. In a second study, birds that received DP living together with a group inoculated with SE at third day old, showed 7.5% infection in LS at 13 day of age and 12.5% at 15 day. Whereas, SE inoculated group had 75% and 57.5% of SE isolates, respectively. A third group, living with the last two, without DP or SE showed 27.5% of SE in LS at 13 day, and only 10% at 15 day of age. DP group at 13 day of age, showed a decrease of 75% of SE colonization at cecal tonsils (CT), instead, SE inoculated group was 100% colonized; at 15 day of age, DP decreased 51.4% of SE colonization in CT, while control group showed a decrease of 42.5%, and 68.6% of SE in CT at 13 and 15 days, respectively. In a third study, a DP booster group was dosed three times, at 14 days of age, it had only 4.5% of SE isolates from LS. Birds without DP showed 34.6% of SE isolates, and the group inoculated with only one dose had 17.2% of SE positive birds. DP booster group showed 22.7% of SE in CT, the group with one dose had 62% of SE isolates; birds without DP decreased only 3.9% of SE colonization. DP showed greater margin of protection, decreased horizontal transmission of SE PT13^A in LS and CT, and it has good transmission potential. DP booster treatment was better than only one dose. DP is a good alternative for SE prevention and eradication in commercial poultry.

Keywords : Competitive Exclusion; Food Safety; Foodborne Illness; Probiotics; Newly Hatched Chicks.

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