Abstract

DELGADO TIBURCIO, Guadalupe Adilia. Effect of three oxygen levels in culture atmosphere and addition of commercial antioxidant in the development of bovine embryos produced in vitro. Acta univ [online]. 2018, vol.28, n.2, pp.53-57. ISSN 2007-9621.  https://doi.org/10.15174/au.2018.1769.

The aim of the present study was to evaluate the effect of three oxygen concentrations in the incubation environment and the use of a commercial antioxidant in semi-defined, sequential culture media on the in vitro production of bovine embryos. After in vitro fertilization, the presumptive zygotes were randomly assigned to six treatments under factorial arrangement: with and without the antioxidant compound (Sigma antioxidant supplement®), under three oxygen concentrations (2%, 5% and 20%) in the incubation atmosphere. The results were analyzed by Análisis de Varianza (Anova), using Tukey test (p < 0.05) to compare treatments. Reduced oxygen (2%), in the incubation environment, had a positive effect on blastocysts and morula production percentage, and cell number (p < 0.05). It is concluded that there are positive effects in the use of reduced oxygen (2%).

Keywords : in vitro production; bovine embryos; antioxidant; ROS.

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