Abstract

PARAVANI, Enrique V. et al. Determination of the concentration of glyphosate in water by the enzyme-linked immunosorbent assay (ELISA). Rev. Int. Contam. Ambient [online]. 2016, vol.32, n.4, pp.399-406. ISSN 0188-4999.  https://doi.org/10.20937/rica.2016.32.04.03.

Herbicides are potential sources of pollution; water being one of the most vulnerable components of the environment. Glyphosate (N-phosphonomethyl glycine) is the most widely used herbicide worldwide. Currently, the information on its effect on water is insufficient, mainly because of the high costs of lab tests. The objectives of this study were to evaluate the use of the ELISA technique as a quick tool for monitoring water quality and to quantify the concentration of glyphosate through the technique of UHPLC-MS/MS. When ELISA positive values determined glyphosate, quantification by UHPLC-MS/MS technique is required to determine the exact concentration of glyphosate in the samples. The results suggest that the ELISA technique is feasible to be implemented in water monitoring programs, providing a screening method that allows detecting qualitatively the presence or absence of glyphosate.

Keywords : contamination; glyphosate; quantification; immunoassay.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )