Abstract

CARREON-ABUD, Yazmín et al. Isolation and propagation of pure cultures of arbuscular mycorrhizal fungi from avocado orchards by the minirhizotron technique. Rev. Mex. Mic [online]. 2013, vol.37, pp.29-39. ISSN 0187-3180.

The aim of this study was to isolate arbuscular mycorrhizal fungi (AMF) from soils of avocado orchards with different agricultural management and to propagate them through the minirhizotron technique. Soil was extracted directly from the study areas, using a completely randomized design and quantifying AMF species richness. Three of the most abundant species, Rhizophagus aff. intraradices, Glomus sp. 1 and Sclerocystis rubiformis were propagated by the minirhizotron technique, assessing mycorrhizal colonization after 20 and 40 days. This system proved to be most effective for propagation and mycorrhization of Sclerocystis rubiformis (18.5%) and Rhizhophagus aff. intraradices and Glomus sp. 1 (7.7%). Minirhizotrons with a consortium of Glomus spp. and Rhizophagus. aff. intraradices and a monospecific culture of Sclerocystis rubiformis were then transplanted to trap pots, for a period of four months. In the minirhizotron culture, the consortium was highly infective from the early stages, reaching 95% of mycorrhizal colonization in comparison with the pure culture of Sclerocystis rubiformis who showed percentages of 58% at the end of the experiment. Therefore the minirhizotron system used proved to be effective in the spread of mono- or multi-specific spore cultures in vivo.

Keywords : monospecific culture; infectivity.

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