Revista mexicana de micología
Print version ISSN 0187-3180
DIAZ, Gisela; FLORES, Roberto and HONRUBIA, Mario. Description of mycelial cultures of Boletales (Boletus of the edulis group, Boletellus and Suillus) from Guatemala and primordium formation of B. edulis in pure culture. Rev. Mex. Mic [online]. 2009, vol.30, pp.1-7. ISSN 0187-3180.
The morphological features of 7 mycelial culture fungal strains belonging to Boletales from Guatemala (2 of Boletus edulis, 1 of B. pinophilus, 1 of B. luteoloincrustatus, 1 of Boletellus russelii, 1 of Suillus tomentosus, 1 of S. bovinus) and 3 fungal strains from Spain (2 of Boletus edulis, 1 of B. pinophilus) are described. They were grown in MNM, Moser B, Gamborg and Pachlewski culture media. The maximal growth was obtained with MNM and Moser medium. Boletus mycelia of the edulis group were similar among them. B. luteoloincrustatus mycelia were darker in colour than B. edulis and B. pinophilus. Suillus tomentosus have a faster growth than the other strains tested in MNM medium. Primordia of B. edulis SO 18 with rudimentary stipe and pileus were obtained in bottles with peat-vermiculite and MNM medium but not in tubes with PDA, at different pH levels.
Keywords : mycelial growth; edible fungi; mycorrhizas; wild strains.