Abstract

JIMENEZ-DELGADILLO, Rocío et al. Effect of pH and temperature on the growth and antagonistic activity of Bacillus subtilis on Rhizoctonia solani. Rev. mex. fitopatol [online]. 2018, vol.36, n.2, pp.256-275. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.1711-3.

In this study, the effects of environmental conditions (pH and temperature) on the growth rate and the inhibitory effect of different strains of Bacillus subtilis on Rhizoctonia solani were evaluated. Strains of B. subtilis were grown in potato infusion broth. The physiological behavior and the growth rate (dN / dt) of the strains were analyzed under different pH and temperature conditions. The strains behaved differently, which allowed establishing optimum and suboptimal growth conditions for each strain. Cell-free supernatants obtained under different growth conditions were used in a quantitative test of in vitro antagonist activity against R. solani. This study showed that the inhibitory effect of the strains occurs mainly in the stationary phase.

Keywords : biocontrol; growth rate; antagonism; physiological state.

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