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Revista mexicana de fitopatología

On-line version ISSN 2007-8080Print version ISSN 0185-3309


ORNELAS-OCAMPO, Katya et al. Variability and symptoms caused by Iris yellow spot virus in Nicotiana benthamiana. Rev. mex. fitopatol [online]. 2018, vol.36, n.1, pp.131-140. ISSN 2007-8080.

In onion Allium cepa crops in the state of Morelos, Mexico, typical and severe symptoms associated with Iris yellow spot virus (IYSV) are observed. In this work the alterations caused by IYSV isolates from typical and severe symptoms in Nicotiana benthamiana, the differences in the N gene and their phylogeny were studied. Four typical and five severe isolates mechanically inoculated caused systemic infection. Severe isolates caused more severe symptoms in bioclimatic chamber. The N gene sequence of both isolates had 98-99% identity with the IYSV nucleoprotein and no changes in nucleotide sequence between them were observed. Both isolates were grouped with the IYSVBR genotype and had greater similarity with those reported in Canada and the United States.

Keywords : Tospovirus; phylogeny; genomic changes.

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