Abstract

PENUELAS-RUBIO, Ofelda et al. Larrea tridentata extracts as an ecological strategy against Fusarium oxysporum radicis-lycopersici in tomato plants under greenhouse conditions. Rev. mex. fitopatol [online]. 2017, vol.35, n.3, pp.360-376. ISSN 2007-8080.  https://doi.org/10.18781/r.mex.fit.1703-3.

The antifungal ability of extracts of Larrea tridentata in the inhibition of Fusarium oxysporum radicis-lycopersici in vitro and in vivo was evaluated. Once the Fusarium strain was characterized, the methodology of poisoned culture medium was used to determine the inhibition of mycelial growth at various concentrations (0-2000 ppm) and solvents (dichloromethane, methanol, ethanol and water). In the in vivo assay, ten treatments were evaluated: 2000 and 3000 ppm for DCM and EtOH, 3000 and 6000 ppm for MeOH and H2O and two controls; one plant as experimental unit and ten replicates. Plant height, leaf number, chlorophyll, severity, as well as incidence and survival were determined in tomato plants inoculated with the fungus and different extracts of L. tridentata. The extracts with a higher percentage of inhibition and vegetative development are dichloromethane at 3000 ppm and methanol at 4000 ppm, both in vitro and in vivo. Extracts of Larrea tridentata can be considered as part of an integrated management plan for the control of crown rot in tomatoes caused by Fusarium oxysporum radicis-lycopersici.

Keywords : biofungicide; protected agriculture; integrated management; Creosote bush.

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