Abstract

LOPEZ-ESTRADA, Martha Elena et al. Management of mango (Mangifera indica L.) seedlings obtained in vitro for plant health studies. Rev. mex. fitopatol [online]. 2008, vol.26, n.2, pp.184-187. ISSN 2007-8080.

In vitro mango seedlings free of external and seed-transmitted contaminants were obtained from immature embryos. Then, they were transplanted to soil using only sterilized materials. Mango embryos 45-60 days old were plated in modified B5 medium; and after seedlings attained 10 cm height, they were transplanted to soil + expanded perlite in specially designed plastic containers. Plants were irrigated with nutritive universal solution. During the in vitro stage 10% contamination with Penicillium, Aspergillus, Xanthomonas and Cladosporium was observed. Contamination in plastic containers gradually reached 15% after five months. Cladosporium, Alternaria, Aspergillus, Rhizopus, and Fusarium were identified in the substrate, whereas Helminthosporium, Curvularia, Penicillium, and Syncephalastrum appeared in the plastic containers.

Keywords : Embryo culture; contaminants; substrate; micropropagation; disinfection.

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