Abstract

VALVERDE, Rodrigo A.  and  GUTIERREZ, Dina L.. Molecular and Biological Properties of a Putative Partitivirus from Jalapeño Pepper (Capsicum annuum L.). Rev. mex. fitopatol [online]. 2008, vol.26, n.1, pp.1-6. ISSN 2007-8080.

Screening 50 greenhouse grown Jalapeño M (JM) (Capsicum annuum) pepper plants for the presence of pepper cryptic virus - 1 (PCV-1) by dsRNA extraction and analysis by electrophoresis, resulted in two JM lines. One was PCV-1 free and designated JM-N. The other one was PCV-1 positive and designated JM-P. Both lines were susceptible to natural infection by Cucumber mosaic virus and its satellite RNA. PCV-1 was not graft transmitted but it was transmitted both paternally (pollen) and maternally (ovule). Partial sequence of the RNA-dependent RNA polymerase gene was obtained from three overlapping cDNA clones derived from PCV-1 dsRNA. A search for aminoacid sequence similarity using the NCBI BLAST resulted in significant alignments with the RNA dependent RNA polymerase of Beet cryptic virus -3 and several putative members of the family Partitiviridae. Data presented indicate that PCV-1 is a distinct species of the family Partitiviridae.

Keywords : Cryptic virus; Cucumber mosaic virus; Beet cryptic virus-3; pepper cryptic virus-1; double-stranded RNA.

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