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Salud Pública de México
Print version ISSN 0036-3634
Abstract
FLORES-PRIMO, Argel et al. Growth and survival of total and pathogenic Vibrio parahaemolyticus in American oyster (Crassostrea virginica) under cold storage. Salud pública Méx [online]. 2015, vol.57, n.3, pp.211-218. ISSN 0036-3634.
Objective. To quantify Vibrio parahaemolyticus densities in American oyster (Crassostrea virginica) under cold storage. Materials and methods. 320 oysters were stored at 7°C for nine days and total and pathogenic densities were determined by the NMP-PCR methodology. Results. V. parahaemolyticus tlh+ densities were observed on 0,3, and 6 days of storage at 1.134, 2.764 and 0.785 log10NMP/g, respectively, and pathogenic density trh+ on 0 and 3 days at 0.477 and 0.519 log10NMP/g, respectively; the pathogenic densities tdh+ (0.519 log10NMP/g), tdh+/trh+ (0.519 log10NMP/g), and tdh+orf8+ (-0.444 log10NMP/g) were detected on day 3 of storage. Conclusion.The results suggest that V. parahaemolyticus growth and pathogenic genes occurrence at 7°C involve changes in the genetic expression as a cold shock response, favoring V. parahaemolyticus survival and virulence, representing a health risk.
Keywords : Vibrio parahaemolyticus; oyster; pathogenicity; refrigeration; Mexico.