Abstract

GARZA-GARCIA, Jorge Alfredo de la et al. Frequency of contamination and serovars of Salmonella enterica and Escherichia coli in an integrated cattle slaughtering and deboning operation. Rev. mex. de cienc. pecuarias [online]. 2020, vol.11, n.4, pp.971-990.  Epub Mar 02, 2021. ISSN 2448-6698.  https://doi.org/10.22319/rmcp.v11i4.5111.

This study aimed to determine the frequency of contamination and serovar diversity of Salmonella enterica (SE) and Escherichia coli (EC) in different stages of cattle slaughtering and deboning processes. Fecal, carcass, and primal cut (100 of each type) samples were collected in a Federally Inspected slaughterhouse in Mexicali, Baja California. EC was not analyzed in fecal samples because it is part of the gut microbiota. Strain identity was confirmed by biochemical methods and PCR, using the taxonomic genes invA and gadA for SE and EC, respectively. In EC, the presence of genes associated with the main pathotypes was also investigated. SE had a 34 % frequency in fecal samples, 3% in carcasses, and 2% in cuts, while Montevideo was the predominant serovar (72.5 % of the total strains). EC was detected in carcasses (34 %) and cuts (11 %) at an average concentration of 0.012 and 0.33 log CFU cm-2, respectively. Although several of the identified EC serovars were associated with enterotoxigenic or Shiga toxin-producing strains, none carried the virulence factors typically observed in these pathotypes. In summary, beef carcasses and cuts are not a relevant source of EC pathogenic strains. However, beef is an important reservoir of SE, which represents a public health risk. Genomic studies are required on the virulence profile and genes of SE strains commonly associated with subclinical infections and isolated from apparently healthy animals.

Keywords : Escherichia coli; Salmonella spp.; Cattle; Slaughter; Serovars; Pathotypes.

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